Symposium B1
Towards understanding compound disposition at ever higher spatial resolution by label free methods

Monday 22 May 2017
15:00-18:00
Stockholmsmässan : K2

Dedicated to the memory of Professor Tsutomu Masujima (1949-2017) – a truly pioneering scientist who strove to do what others could not do.

Introduction

All too frequently we do not know enough about the likely disposition of compounds early in the discovery phase with the result there is a greater emphasis placed on potency at the target which is more easily optimised. This session will focus on contemporary methods for increasing the understanding of cellular and subcellular compound disposition using unlabelled drug, suitable for use in the lead optimisation phase.

Co-Chair(s): Mike Hann (UK), Paulina Rakowska (UK)

Programme

15:00 – 15:05  Introductory remarks
Mike Hann, GSK, UK

15:05 – 15:35 Intracellular unbound drug concentrations: methodology and application for understanding cellular drug exposure
André Mateus, University of Uppsala, Sweden

15:35 – 16:05 A modular probe strategy for drug localization, target identification and target engagement measurement on single cell level
Anna Rutkowska-Klute, Cellzome, Germany

16:05 – 16:35 3D label-free imaging of drug uptake into cells and bacteria using secondary ion mass spectrometry
Paulina Rakowska,National Physical Laboratory, UK

16:35 – 16:50 BREAK

16:50 – 17:20 Utilizing Label Free High Content Imaging Methods to Span the Gap from Discovery to the Clinic
Zane Arp, GSK, USA

17:20 – 17:50 Single-cell Drug Discovery, Diagnosis and Personalized Medicine by Live-MS
Ahmed Ali, RIKEN Quantitative Biology Center, Japan

17:50 – 18:00  Concluding remarks